Low-velocity collision behaviour of clusters composed of sub-mm sized dust aggregates

The experiments presented aim to measure the outcome of collisions between sub-mm sized protoplanetary dust aggregate analogues. We also observed the clusters formed from these aggregates and their collision behaviour. The experiments were performed at the drop tower in Bremen. The protoplanetary dust analogue materials were micrometre-sized monodisperse and polydisperse SiO$_2$ particles prepared into aggregates with sizes between 120~$\mu$m and 250~$\mu$m. One of the dust samples contained aggregates that were previously compacted through repeated bouncing. During three flights of 9~s of microgravity each, individual collisions between aggregates and the formation of clusters of up to a few millimetres in size were observed. In addition, the collisions of clusters with the experiment cell walls leading to compaction or fragmentation were recorded. We observed collisions amongst dust aggregates and collisions between dust clusters and the cell aluminium walls at speeds ranging from about 0.1 cm/s to 20 cm/s. The velocities at which sticking occurred ranged from 0.18 to 5.0 cm/s for aggregates composed of monodisperse dust, with an average value of 2.1 cm/s for reduced masses ranging from 1.2x10-6 to 1.8x10-3 g with an average value of 2.2x10-4 g. From the restructuring and fragmentation of clusters composed of dust aggregates colliding with the aluminium cell walls, we derived a collision recipe for dust aggregates ($\sim$100 $\mu$m) following the model of Dominik \& Thielens (1997) developed for microscopic particles. We measured a critical rolling energy of 1.8x10-13 J and a critical breaking energy of 3.5x10-13 J for 100 $\mu$m-sized non-compacted aggregates.Comment: 12 pages, 13 figure

Submillimetre-sized dust aggregate collision and growth properties

The collisional and sticking properties of sub-mm-sized aggregates composed of protoplanetary dust analogue material are measured, including the statistical threshold velocity between sticking and bouncing, their surface energy and tensile strength within aggregate clusters. We performed an experiment on the REXUS 12 suborbital rocket. The protoplanetary dust analogue materials were micrometre-sized monodisperse and polydisperse SiO2 particles prepared into aggregates with sizes around 120 $\mu$m and 330 $\mu$m, respectively and volume filling factors around 0.37. During the experimental run of 150 s under reduced gravity conditions, the sticking of aggregates and the formation and fragmentation of clusters of up to a few millimetres in size was observed. The sticking probability of the sub-mm-sized dust aggregates could be derived for velocities decreasing from 22 to 3 cm/s. The transition from bouncing to sticking collisions happened at 12.7 cm/s for the smaller aggregates composed of monodisperse particles and at 11.5 and 11.7 cm/s for the larger aggregates composed of mono- and polydisperse dust particles, respectively. Using the pull-off force of sub-mm-sized dust aggregates from the clusters, the surface energy of the aggregates composed of monodisperse dust was derived to be 1.6x10-5 J/m2, which can be scaled down to 1.7x10-2 J/m2 for the micrometre-sized monomer particles and is in good agreement with previous measurements for silica particles. The tensile strengths of these aggregates within the clusters were derived to be 1.9 Pa and 1.6 Pa for the small and large dust aggregates, respectively. These values are in good agreement with recent tensile strength measurements for mm-sized silica aggregates. Using our data on the sticking-bouncing threshold, estimates of the maximum aggregate size can be given. For a minimum mass solar nebula model, aggregates can reach sizes of 1 cm.Comment: 21 pages (incl. 6 pages of appendix), 23 figure

Non-linear photochemical pathways in laser induced atmospheric aerosol formation

We measured the chemical composition and the size distribution of aerosols generated by femtosecond-Terawatt laser pulses in the atmosphere using an aerosol mass spectrometer (AMS). We show that nitric acid condenses in the form of ammonium nitrate, and that oxidized volatile organics also contribute to particle growth. These two components account for two thirds and one third, respectively, of the dry laser-condensed mass. They appear in two different modes centred at 380 nm and 150 nm. The number concentration of particles between 25 and 300 nm increases by a factor of 15. Pre-existing water droplets strongly increase the oxidative properties of the laser-activated atmosphere, substantially enhancing the condensation of organics under laser illumination.Comment: 19 pages, 5 figure

Same ammo, different weapons: Enzymatic extracts from two apple genotypes with contrasted susceptibilities to fire blight (Erwinia amylovora) differentially convert phloridzin and phloretin in vitro

The necrogenic bacterium Erwinia amylovora responsible for the fire blight disease causes cell death in apple tissues to enrich intercellular spaces with nutrients. Apple leaves contain large amounts of dihydrochalcones (DHCs), including phloridzin and its aglycone phloretin. Previous work showed an important decrease in the constitutive DHCs stock in infected leaves, probably caused by transformation reactions during the infection process. At least two flavonoid transformation pathways have been described so far: deglucosylation and oxidation. The aim of the present study was to determine whether DHCs are differentially converted in two apple genotypes displaying contrasted susceptibilities to the disease. Different analyses were performed: i) enzymatic activity assays in infected leaves, ii) identification/quantification of end-products obtained after in vitro enzymatic reactions with DHCs, iii) evaluation of the bactericidal activity of end-products. The results of the enzymatic assays showed that deglucosylation was dominant over oxidation in the susceptible genotype MM106 while the opposite was observed in the resistant genotype Evereste. These data were confirmed by LC–UV/Vis–MS analysis of in vitro reaction mixtures, especially because higher levels of o-quinoid oxidation products of phloretin were measured by using the enzymatic extracts of Evereste infected leaves. Their presence correlated well with a strong bactericidal activity of the reaction mixtures. Thus, our results suggest that a differential transformation of DHCs occur in apple genotypes with a potential involvement in the establishment of the susceptibility or the resistance to fire blight, through the release of glucose or of highly bactericidal compounds respectively

Space-mapping techniques applied to the optimization of a safety isolating transformer

Space-mapping optimization techniques allow to allign low-fidelity and high-fidelity models in order to reduce the computational time and increase the accuracy of the solution. The main idea is to build an approximate model from the difference of response between both models. Therefore the optimization process is computed on the surrogate model. In this paper, some recent approaches of space-mapping techniques such as agressive-space-mapping, output-mapping and manifold-mapping algorithms are applied to optimize a safety insulating transformer. The electric, magnetic and thermal phenomena of the device are modeled by an analytical model and a 3D finite element model. It is considered as a benchmark for multi-level optimization to test different algorithms

T3SS-dependent differential modulations of the jasmonic acid pathway in susceptible and resistant genotypes of Malus spp. challenged with Erwinia amylovora

Fire blight is a bacterial disease of Maloideae caused by Erwinia amylovora (Ea). This necrogenic enterobacterium uses a type III secretion system (T3SS) to inject type III effectors into the plant cells to cause disease on its susceptible hosts, including economically important crops like apple and pear. The expressions of marker genes of the salicylic acid (SA) and jasmonic acid (JA) defense regulation pathways were monitored by RT-qPCR in leaves of two apple genotypes, one susceptible and one resistant, challenged with a wild type strain, a T3SS-deficient strain or water. The transcriptional data taken together with hormone level measurements indicated that the SA pathway was similarly induced in both apple genotypes during infection by Ea. On the contrary, the data clearly showed a strong T3SS-dependent down-regulation of the JA pathway in leaves of the susceptible genotype but not in those of the resistant one. Accordingly, methyl-jasmonate treated susceptible plants displayed an increased resistance to Ea. Bacterial mutant analysis indicated that JA manipulation by Ea mainly relies on the type III effector DspA/E. Taken together, our data suggest that the T3SS-dependent down-regulation of the JA pathway is a critical step in the infection process of Malus spp. by Ea

Histolocalization and physico-chemical characterization of dihydrochalcones: Insight into the role of apple major flavonoids

Flavonoids, like other metabolites synthesized via the phenylpropanoid pathway, possess a wide range of biological activities including functions in plant development and its interaction with the environment. Dihydrochalcones (mainly phloridzin, sieboldin, trilobatin, phloretin) represent the major flavonoid subgroup in apple green tissues. Although this class of phenolic compounds is found in very large amounts in some tissues (≈200 mg/g of leaf DW), their physiological significance remains unclear. In the present study, we highlight their tissue-specific localization in young growing shoots suggesting a specific role in important physiological processes, most notably in response to biotic stress. Indeed, dihydrochalcones could constitute a basal defense, in particular phloretin which exhibits a strong broad-range bactericidal and fungicidal activity. Our results also indicate that sieboldin forms complexes with iron with strong affinity, reinforcing its antioxidant properties and conferring to this dihydrochalcone a potential for iron seclusion and/or storage. The importance of localization and biochemical properties of dihydrochalcones are discussed in view of the apple tree defense strategy against both biotic and abiotic stresses

On the groove pressing of Ni-W alloy: microstructure, texture and mechanical properties evolution

International audienceThe microstructure, texture and mechanical properties of the Ni-14%W(wt.%) alloy with two different initial grain sizes and textures were investigated after groove pressing (GP) at 450 °C to 4 cycles using Electron Back Scatter Diffraction (EBSD) and microhardness measurements. The initial first series was characterized by small equiaxed grains and Cube dominant texture component. The second series has elongated grains and β-fiber texture. EBSD analysis has shown that GP processing led to a slight refinement (less than 15%) of equiaxed grains in series I while greater refinement (~55%) of the mean spacing along normal direction was observed in series II. The texture did not drastically change from the initial ones and was characterized by the weakening of the Cube component in series I and rapid decrease of Copper component for series II. GP processing reduces very slightly the plastic anisotropy of the alloy with initial elongated granular microstructure

Mutational analysis of a predicted double β-propeller domain of the DspA/E effector of Erwinia amylovora

The bacterium Erwinia amylovora causes fire blight, an invasive disease that threatens apple trees, pear trees and other plants of the Rosaceae family. Erwinia amylovora pathogenicity relies on a type III secretion system and on a single effector DspA/E. This effector belongs to the widespread AvrE family of effectors whose biological function is unknown. In this manuscript, we performed a bioinformatic analysis of DspA/E- and AvrE-related effectors. Motif search identified nuclear localization signals, peroxisome targeting signals, endoplasmic reticulum membrane retention signals and leucine zipper motifs, but none of these motifs were present in all the AvrE-related effectors analysed. Protein threading analysis, however, predicted a conserved double β-propeller domain in the N-terminal part of all the analysed effector sequences. We then performed a random pentapeptide mutagenesis of DspA/E, which led to the characterization of 13 new altered proteins with a five amino acids insertion. Eight harboured the insertion inside the predicted β-propeller domain and six of these eight insertions impaired DspA/E stability or function. Conversely, the two remaining insertions generated proteins that were functional and abundantly secreted in the supernatant suggesting that these two insertions stabilized the protein